DFCI shivdasani SAGE
Mammalian Fetal Gut Development

In generic development of the vertebrate gastrointestinal (GI) tract, the rostrally placed stomach differentiates early from the caudally located intestine. The molecular mechanisms that underlie this differentiation are poorly understood. Using a systematic approach of mRNA expression profiling, we are attempting to obtain an overview of regional gene expression in the fetal GI tract. These data should provide insight into the molecular distinction between discrete regions in the developing gut and help identify candidate mediators of the relevant cellular transitions. We have constructed SAGE (Serial Analysis of Gene Expresion) libraries from the mouse stomach and small intestine at post-coital day (E) 12.5. The results from this analysis are presented here in a readily searchable form. To see a comparison between mRNA expression at different stages in development of the fetal mouse intestine, please visit http://genome.dfci.harvard.edu/GutSAGE.

Further analysis of the comparison between early stomach and intestine gene expression is published in the following report: Kim B-M, Buchner G, Miletich I, Sharpe PT, Shivdasani RA. The stomach mesenchymal transcription factor Barx1 specifies gastric epithelial identity through inhibition of transient Wnt signaling. Dev Cell 2005; In Press.


Fetal (post-coital) age and organ E12.5 Intestine E12.5 Stomach
SAGE tags sequenced 65585 65414
Unique SAGE tags 12168 10319


These SAGE libraries are available for queries and comparisons

Click Here to Download the Whole Dataset!

Please send comments to: ramesh_shivdasani@dfci.harvard.edu